Media playback is not supported on this device Analysis: The surprise loss of the sisters at Wimbledon – BBC Sport’s Roger Draper
The Williams sisters are out of the US Open.
Six-time winner Serena and her younger sister Venus have opted not to play at Flushing Meadows, which starts on 28 August.
Wimbledon winner Garbine Muguruza and German Julia Goerges will instead compete for the women’s title at Flushing Meadows.
Meanwhile, Novak Djokovic and Rafael Nadal will compete in the men’s doubles at the US Open as they both recover from injuries.
Tournament director David Brewer said: “This year’s field has been enhanced by the inclusion of two former champions in the women’s field, and by the outstanding efforts of the current players whose Grand Slam aspirations will now be served by playing at the US Open.”
Venus Williams, 36, who last won the US Open in 2008, had to retire from her US Open qualifying match on Tuesday and will also miss Wimbledon as she takes maternity leave.
Serena Williams, 34, lost in the first round of Wimbledon earlier this month.
The pair will play in the final of this month’s Cincinnati Open, their second tournament back on the WTA Tour.